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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H⁺

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Biochemistry

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JoVE Journal Biochemistry

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H⁺

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H⁺

DOI:

10.3791/57979-v

•

08:26 min

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June 25, 2018

•

Ya-Min Du, Shen-Li Zheng, Li Liu, Josef Voglmeir, Gabriel Yedid

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology,Nanjing Agricultural University, ²College of Life Science,Nanjing Agricultural University

Chapters

00:04Title
00:41Isolation of Protein from Radish and Preparation of N-Glycans
02:35Purification and Fluorescence Derivatization of N-Glycans
04:47HILIC-UPLC profiling of N-Glycans and MALDI-TOF MS Analysis
06:24Results: N-glycan Analysis
06:54Conclusion

Summary

Automatic Translation

We describe a simple and rapid method for the preparation and analysis of N-glycans from different cultivars of radish (Raphanus sativus).

Tags

Keywords: N-glycans Raphanus Sativus PNGase H+Plant Glycoscience Protein Isolation TCA Precipitation N-glycan Release Solid-phase Extraction Fluorescence Derivatization

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