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Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography
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Bioengineering
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JoVE Journal Bioengineering
Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography

Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography

DOI:
10.3791/58040-v

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11:12 min

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October 03, 2018

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, , , , ,
1Department of Cardiology,Guangdong Academy of Medical Sciences, Guangdong General Hospital, 2Medical Research Center,Guangdong Academy of Medical Sciences, Guangdong General Hospital, 3Institute of Geriatric medicine,Guangdong Academy of Medical Sciences, Guangdong General Hospital

Chapters

  • 00:04Title
  • 00:52Degradable Polyglycolic Acid (PGA) Scaffold-based Tissue-engineered Vessels Culture
  • 05:41Performing Optical Imaging with OCT and Image Analysis
  • 08:10Harvest of the TEBVs and Tissue Processing
  • 09:14Results: Characterization of Biodegradable Polymeric Scaffold-Based TEBVs and Remodeling Process through OCT Imaging
  • 10:36Conclusion

Summary

Automatic Translation

Automatic Translation

A step by step protocol for nondestructive and long-period monitoring the process of vascular remodeling and scaffold degradation in real-time culture of biodegradable polymeric scaffold-based tissue-engineered blood vessels with pulsatile stimulation using optical coherence tomography is described here.

Tags

Nondestructive MonitoringDegradable ScaffoldOptical Coherence TomographyScaffold FabricationBioreactor AssemblyHuman Umbilical Artery Smooth Muscle CellsCell Seeding

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