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En høj overførselshastighed, High-indhold, væskebaseret C. elegans Pathosystem
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A High-throughput, High-content, Liquid-based C. elegans Pathosystem
DOI:

09:44 min

July 01, 2018

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Chapters

  • 00:04Title
  • 00:25Preparation and Maintenance of P. aeruginosa and RNAi Bacteria
  • 02:34Maintenance and Preparation of C. elegans
  • 03:21Liquid Killing Assay Setup
  • 05:56Adaptation for Screening Multiple C. elegans Strains or Knockdowns
  • 07:06Results: C. elegans-P. aeruginosa Liquid Killing Pathosystem
  • 08:37Conclusion

Summary

Automatic Translation

Her beskriver vi en protokol, der er en fleksibel, hele vært, high-indhold screeningsværktøj, der kan udnyttes til at studere vært-patogen interaktioner og bruges for drug discovery.

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