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Biochemistry
Author Produced

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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
 

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

Article DOI: 10.3791/58076-v 08:08 min September 11th, 2018
September 11th, 2018

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Summary

Here, we present a protocol to quantitatively determine phycobiliprotein content in the cyanobacterium Synechocystis using a spectrophotometric method. The extraction procedure was also successfully applied to other cyanobacteria and algae strains; however, due to variations in pigment absorption spectra, it is necessary to test the spectrophotometric equations for each strain individually.

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Keywords: Spectrophotometric Determination Phycobiliproteins Cyanobacterium Synechocystis Phycocyanin Allophycocyanin Pigment-protein Complexes Light-harvesting Antennae Cyanobacteria Productivity Extraction Freeze-drying Homogenization
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