Journal
/
Immunology and Infection
/
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

DOI:
10.3791/58083-v

•

09:45 min

•

August 24, 2018

•

,
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine,University of California, Davis

Chapters

  • 00:04Title
  • 00:34Neutrophil Isolation
  • 03:03Live Neutrophil Imaging and Neutrophil Extracellular Trap (NET) Detection and Quantification
  • 07:18Results: Representative Canine Neutrophil Imaging and NET Detection
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

We describe methods to isolate canine neutrophils from whole blood and visualize NET formation in live neutrophils using fluorescence microscopy. Also described are protocols to quantify NET formation and citrullinated histone H3 (citH3) expression using immunofluorescence microscopy.

Tags

In VitroCanineNeutrophilExtracellular TrapFormationFluorescence MicroscopyNETPeroxidaseCitrullinated Histone H3BloodJugular VeinAnticoagulantDextranDensity Gradient CentrifugationErythrocytesPolymorphonuclear CellsCell Culture BufferTrypan BluePoly-L-lysineFluorescence Microscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58083?article_type=v

Neutrophil Extracellular Traps: How to Generate and Visualize Them
check_url/58083?article_type=v

Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
check_url/58083?article_type=v

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
check_url/58083?article_type=v

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
check_url/58083?article_type=v

Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
check_url/58083?article_type=v

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
check_url/58083?article_type=v

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
check_url/58083?article_type=v

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
check_url/58083?article_type=v

Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy
check_url/58083?article_type=v

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Read Article