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Visualisere genetiske varianter, korte mål og punktmutationer i forbindelse morfologiske væv med en RNA In Situ hybridisering Assay
JoVE Journal
Biology
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JoVE Journal Biology
Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Visualisere genetiske varianter, korte mål og punktmutationer i forbindelse morfologiske væv med en RNA In Situ hybridisering Assay

DOI:
10.3791/58097-v

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10:57 min

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August 14, 2018

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, , , , , , , , , ,
1Advanced Cell Diagnostics, Inc

Chapters

  • 00:04Title
  • 00:41Tissue Preparation
  • 01:30Equipment and Reagent Preparation
  • 03:24Sample Deparaffinization, Dehydration, and Pre-treatment
  • 05:26Probe Hybridization, Signal Amplification, and Detection
  • 07:18Counterstaining, Slide Mounting, and Visualization
  • 08:18Results: Detection of Splice Variants, Short Sequences, and Point Mutation In Situ
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

Her, beskriver vi en i situ hybridisering analyse, som gør det muligt for følsom og specifik påvisning af sekvenser så kort som 50 nukleotider med enkelt-nukleotid opløsning på én celle-niveau. Analysen, som kan udføres manuelt eller automatisk, kan aktivere visualisering af splice varianter, korte sekvenser og mutationer inden konteksten væv.

Tags

Keywords: Genetic VariantsShort TargetsPoint MutationsRNA In Situ HybridizationCell Type ExpressionSplice VariantsNeuroscienceOncologyExon JunctionsSensitivitySpecificitySingle CellsFormalin FixationParaffin EmbeddingMicrotome SectioningTarget RetrievalWash BufferHematoxylin CounterstainProbe HybridizationAmplification Reagents

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