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Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes
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Chapters
- 00:04Title
- 00:30Data Preparation
- 02:32Cell Counting
- 04:10Measuring Astrocytic Network Area
- 05:04Determining the Main Direction Vector
- 06:52Placing the Analyzed Networks in the Nucleus of Interest
- 09:11Results: Astrocytic Networks Labeled with Biocytin in NVsnpr
- 09:49Conclusion
Here we present a protocol to assess the organization of astrocytic networks. The described method minimizes bias to provide descriptive measures of these networks such as cell count, size, area, and position within a nucleus. Anisotropy is assessed with a vectorial analysis.
