Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Endogenous Protein Tagging in Human Induced…
JoVE Journal
Genetics
Author Produced

This content is Open Access.

JoVE Journal Genetics
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
 

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Article DOI: 10.3791/58130-v • 14:49 min • August 25th, 2018
August 25th, 2018 •
1, 1, 1, 1, 1, 1
1Allen Institute for Cell Science

Chapters

Summary
Automatic Translation

Described here is a protocol for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.

Tags

Keywords: CRISPR/Cas9 Endogenous Protein Tagging Human Induced Pluripotent Stem Cells Ribonucleoprotein Fluorescent Tag FACS Clonal Cell Lines Gene Editing Live Cell Imaging Cellular Structures Cellular Processes
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter