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Korrelationsmaalinger lys Elektron Mikroskopi (CLEM) til sporing og Imaging Viral Protein associeret strukturer i Cryo-immobiliserede celler
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Korrelationsmaalinger lys Elektron Mikroskopi (CLEM) til sporing og Imaging Viral Protein associeret strukturer i Cryo-immobiliserede celler

DOI:
10.3791/58154-v

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09:18 min

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September 07, 2018

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, , , , , , ,
1European Molecular Biology Laboratory, 2Department of Infectious Diseases, Molecular Virology,Heidelberg University, 3Central Facility for Electron Microscopy,Ulm University, 4Heidelberg Partner Site,German Center for Infection Research

Chapters

  • 00:04Title
  • 00:51Patterned Sapphire Disc Preparation, Cell Culture, and Imaging
  • 03:16Cryo-immobilization and Freeze Substitution (FS)
  • 05:24Resin Embedding and Patterned Sapphire Disc Removal from the Polymerized Resin Block
  • 06:43Ultrathin Section Preparation and Imaging via Transmission Electron Microscopy (TEM)
  • 08:06Results: Tracking and Imaging of Viral Protein-associated Structures via Correlative Light Electron Microscopy (CLEM)
  • 08:47Conclusion

Summary

Automatic Translation

Automatic Translation

En korrelationsmaalinger lys elektronmikroskopi (CLEM) metode kan anvendes på billedet virus-induceret intracellulære strukturer via elektronmikroskopi (EM) i celler, der er tidligere vælges ved lysmikroskopi (LM). LM og EM er kombineret som en hybrid imaging tilgang til at opnå en integreret visning af virus-værtssammenspil.

Tags

Keywords: Correlative Light Electron Microscopy (CLEM)Viral ProteinCellular MembranesBio-replicationHigh Pressure FreezingCryo-immobilizationElectron MicroscopyLight MicroscopyFluorescent ProteinsVirus-infected CellsTransfected CellsUltrastructure Analysis

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