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कैफीन निष्कर्षण, एंजाइमी गतिविधि और संयंत्र सेल सस्पेंशन से कैफीन सिंथेस की जीन अभिव्यक्ति
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Chapters
- 00:04Title
- 00:28Caffeine Extraction in Cell Suspension of C. arabica L.
- 00:55Conditions for the Assessment of Caffeine by Thin Layer Chromatography (TLC) Denistometry
- 01:29Ribonucleic Acid (RNA) Isolation
- 02:55Incubation of RNA Transcript with Deoxyribonuclease (DNase)
- 03:59Complementary Deoxyribonucleic Acid (cDNA) Synthesis
- 05:03Real-Time Quantitative PCR (qPCR) to Amplify the Gene CCS1
- 05:43Total Protein of Cell Suspensions of C. arabica L.
- 06:26Enzymatic Assay for Caffeine Synthase
- 07:39Results: Enzymatic Activity of Caffeine Synthase Gene Evaluated via qPCR
- 08:30Conclusion
