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Påvisning af lav kopi nummer integreret Viral DNA dannet af In Vitro Hepatitis B infektion
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
DOI:

11:14 min

November 07, 2018

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Chapters

  • 00:04Title
  • 00:52Cell Infection and DNA Extraction
  • 02:42Inversion of DNA
  • 05:55Nested PCR
  • 08:30PCR Product Isolation and Gel Extraction
  • 09:37Results: Agarose Gel Electrophoresis and BLAST Analysis of InvPCR Product Sequences
  • 10:24Conclusion

Summary

Automatic Translation

Vi beskriver her in vitro- generation af HBV DNA via en Hepatitis B virus infektion system og meget følsomme påvisning af dets (1 – 2 kopier) integration ved hjælp af inverse indlejret PCR.

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