Journal
/
Biochemistry
/
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

DOI:
10.3791/58283-v

•

09:14 min

•

August 20, 2018

•

, ,
1Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences,North Carolina State University

Chapters

  • 00:04Title
  • 00:32Modification of a Baculovirus Expression Vector with either the GP67 Signal Peptideor the Insect Hemolin Signal Peptidefor Plant Protein Secretion Expression
  • 02:24Production and Amplification of Baculovirus Constructs Harboring the Recombinant Protein Expression Cassette
  • 07:02Results: Analysis of Plant Proteins Secreted by Insect Cells
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present the protocols for utilizing the insect cell and baculovirus protein expression system to produce large quantities of plant secreted proteins for protein crystallization. A baculovirus expression vector has been modified with either GP67 or insect hemolin signal peptide for plant protein secretion expression in insect cells.

Tags

Baculovirus Expression VectorsSecreted Plant ProteinsInsect CellsProtein Structure DeterminationRecombinant ProteinsDNA CloningDNA SequencingBacterial TransformationBacmid DNASf9 Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58283?article_type=v

Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
check_url/58283?article_type=v

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
check_url/58283?article_type=v

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
check_url/58283?article_type=v

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
check_url/58283?article_type=v

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
check_url/58283?article_type=v

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
check_url/58283?article_type=v

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
check_url/58283?article_type=v

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
check_url/58283?article_type=v

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
check_url/58283?article_type=v

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Read Article