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Endre Baculovirus uttrykk vektorer å produsere utskilles anlegget proteiner i insekt celler
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Chapters
- 00:04Title
- 00:32Modification of a Baculovirus Expression Vector with either the GP67 Signal Peptideor the Insect Hemolin Signal Peptidefor Plant Protein Secretion Expression
- 02:24Production and Amplification of Baculovirus Constructs Harboring the Recombinant Protein Expression Cassette
- 07:02Results: Analysis of Plant Proteins Secreted by Insect Cells
- 07:57Conclusion
Her presenterer vi protokollene for utnytte insekt celler og baculovirus protein uttrykk systemet å produsere store mengder anlegget utskilles proteiner for protein krystallisering. En baculovirus uttrykk vektor er endret med GP67 eller insekt hemolin signal peptid plante protein sekresjon uttrykk i insekt celler.
