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Generation af menneskelige primordiale kønsceller-lignende celler på overfladen af Embryoid organer fra primet pluripotency induceret pluripotente stamceller
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Generation af menneskelige primordiale kønsceller-lignende celler på overfladen af Embryoid organer fra primet pluripotency induceret pluripotente stamceller

DOI:
10.3791/58297-v

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12:06 min

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January 11, 2019

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, , , ,
1Center for Cancer Research,Massachusetts General Hospital, 2Department of Molecular Genetics,Weizmann Institute of Science

Chapters

  • 00:04Title
  • 01:01Inoculation and Conversion of Primed Pluripotency Human Induced Pluripotent Stem Cells (hiPSCs)
  • 03:40Embryoid Body (EB) Formation Using Microwell Plates
  • 05:52Transfer of EBs to Low Attachment Plates and Generation of hPGCLCs on the Surface of EBs
  • 08:00Immunohistochemical Staining of EBs
  • 09:41Results: Successful Generation of EBs with hPGCLCs on their Surface
  • 11:29Conclusion

Summary

Automatic Translation

Automatic Translation

Primordiale kønsceller (PGCs) er fælles forløbere for både sæd og æg. Menneskelige embryonale PGCs er angivet fra pluripotente epiblast celler gennem interaktioner af cytokiner. Her beskriver vi en 13-dag protokol af inducerende menneskeceller transcriptomally ligner PGCs på overfladen af embryoid organer fra PRIMES-pluripotency induceret pluripotente stamceller.

Tags

Human Primordial Germ Cell-like CellsPrimed-pluripotencyInduced Pluripotent Stem CellsGermline ToxicologyGenomic ImpairmentExtracellular MatrixSingle-cell SuspensionNaive Pluripotency4i MediumReproducibilityCell DensityCell Culture Model

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