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Developmental Biology
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附睾蛋白的合成与分泌分析
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Analysis of Epididymal Protein Synthesis and Secretion
Please note that all translations are automatically generated.
Click here for the English version.
附睾蛋白的合成与分泌分析
DOI:
10.3791/58308-v
•
10:23 min
•
August 25, 2018
•
Wei Zhou
2
,
Petra Sipilä
,
Geoffry N. De Iuliis
2
,
Matthew D. Dun
4
,
Brett Nixon
2
1
Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences
,
University of Newcastle
,
2
Hunter Medical Research Institute
,
3
Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine
,
University of Turku
,
4
School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine
,
University of Newcastle
Chapters
00:04
Title
00:36
Immunofluorescence Staining of the Paraffin-embedded Epididymal Sections
02:44
Isolation of Epididymosomes from the Mouse Caput Epididymis
05:26
Immunofluorescence Staining of mECap18 Cells
06:22
Isolation of Proteins from Conditioned Cell Culture Medium
08:06
Results: Analysis of Epididymal Protein Synthesis and Secretion
09:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们报告动力蛋白的免疫荧光定位, 以说明在石蜡嵌入小鼠附睾切片和永生化附睾细胞系 (mECap18) 的蛋白质检测协议。我们还描述了从附睾液和条件细胞培养基中分离分泌蛋白的协议。
Tags
Epididymal Protein Synthesis
Epididymal Secretion
Sperm Maturation
Sperm Storage
Epididymal Isolation
Immunohistochemistry
Antigen Retrieval
Primary Antibody
Secondary Antibody
Mouse Epididymis
Blood Contamination
BWW Medium
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