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1Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences,University of Newcastle, 2Hunter Medical Research Institute, 3Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine,University of Turku, 4School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine,University of Newcastle
Chapters
- 00:04Title
- 00:36Immunofluorescence Staining of the Paraffin-embedded Epididymal Sections
- 02:44Isolation of Epididymosomes from the Mouse Caput Epididymis
- 05:26Immunofluorescence Staining of mECap18 Cells
- 06:22Isolation of Proteins from Conditioned Cell Culture Medium
- 08:06Results: Analysis of Epididymal Protein Synthesis and Secretion
- 09:45Conclusion
Aqui, nós relatamos a localização de imunofluorescência de Dinamina para ilustrar os protocolos para a detecção de proteínas em seções epidídimo de rato de parafina e aqueles de uma linhagem de células epidídimo imortalizado (mECap18). Também descrevemos os protocolos para o isolamento de proteínas secretoras do epidídimo fluido e mídia celular condicionados.
