/

/

Laminectomy and Spinal Cord Window Implantation in the Mouse

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

Laminectomy and Spinal Cord Window Implantation in the Mouse

Laminectomy and Spinal Cord Window Implantation in the Mouse

DOI:

10.3791/58330-v

•

06:59 min

•

October 23, 2019

•

Elizabeth A. Pietruczyk, Terilyn K.L. Stephen, Simon Alford, Sarah E. Lutz

¹Department of Anatomy and Cell Biology,University of Illinois at Chicago College of Medicine, ²Medical Scientist Training Program,University of Illinois at Chicago College of Medicine

Chapters

00:04Title
00:51Backplate 3D Printing and Laminectomy
03:56Cover Glass Implantation and Imaging
05:46Results: Representative Tight Junction Microvasculature Intravital 2-Photon Microscopic Imaging
06:14Conclusion

Summary

Automatic Translation

This protocol describes implantation of a glass window onto the spinal cord of a mouse to facilitate visualization by intravital microscopy.

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Spinal Cord Transection in the Larval Zebrafish

A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia

Read Article