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3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
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1Departments of Internal Medicine, Hematology/Oncology Division, Rogel Cancer Center,University of Michigan Medical Center, 2Department of Urology, Division of GU Oncology, Rogel Cancer Center,University of Michigan Medical Center, 3Departments of Surgery and Pathology, Perlmutter Cancer Center,NYU Langone Health
Chapters
- 00:04Title
- 01:03Growing Cancer Spheroids
- 02:24Preparing the 3D Culture Chamber
- 03:50Live Cell Time-lapse Imaging
- 04:24Preparation of Sample Block Containing Cancer Spheroids for Frozen Tissue Sectioning
- 05:36Immunofluorescence Imaging for Frozen Sectioned Cancer Spheroids
- 07:18Results: Invasive Bladder Cancer Tumor Spheroids Formed from Cell Lines and Primary Tumors
- 08:45Conclusion
The processes governing bladder cancer invasion represent opportunities for biomarker and therapeutic development. Here we present a bladder cancer invasion model which incorporates 3-D culture of tumor spheroids, time-lapse imaging and confocal microscopy. This technique is useful for defining the features of the invasive process and for screening therapeutic agents.
