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3D-celle kultur System for at studere Invasion og evaluere Therapeutics i blærekræft
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1Departments of Internal Medicine, Hematology/Oncology Division, Rogel Cancer Center,University of Michigan Medical Center, 2Department of Urology, Division of GU Oncology, Rogel Cancer Center,University of Michigan Medical Center, 3Departments of Surgery and Pathology, Perlmutter Cancer Center,NYU Langone Health
Chapters
- 00:04Title
- 01:03Growing Cancer Spheroids
- 02:24Preparing the 3D Culture Chamber
- 03:50Live Cell Time-lapse Imaging
- 04:24Preparation of Sample Block Containing Cancer Spheroids for Frozen Tissue Sectioning
- 05:36Immunofluorescence Imaging for Frozen Sectioned Cancer Spheroids
- 07:18Results: Invasive Bladder Cancer Tumor Spheroids Formed from Cell Lines and Primary Tumors
- 08:45Conclusion
Processer for blære cancer invasion repræsenterer muligheder for biomarkør og terapeutisk udvikling. Her præsenterer vi en blære kræft invasion model, der indeholder 3D-kultur af tumor spheroids, time-lapse billedbehandling og konfokal mikroskopi. Denne teknik er nyttig til at definere funktioner af den invasive proces og for screening terapeutiske agenter.
