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Chapters
- 00:04Title
- 00:34O9-1 Cell Culture
- 01:00Recovery of O9-1 Cells
- 02:59Passage of O9-1 Cells
- 04:27Freezing of O9-1 Cells
- 05:48Manipulation of O9-1 Cells and Differentiation of O9-1 Cells
- 07:11Results: Yap-null Cells Fail to Differentiate into SMA-positive Smooth Muscle Cells
- 07:49Conclusion
O9-1 är en multipotenta mus neural crest cellinje. Här beskriver vi detaljerade stegvisa protokoll för odling O9-1 celler, differentiera O9-1 celler till specifika celltyper och genetiskt manipulera O9-1 celler med siRNA-medierad knockdown eller CRISPR-Cas9 gen editering.
