Journal
/
Developmental Biology
/
Induktion i Endothelial differentiering i hjertets stamceller lavt Serum betingelser
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

Induktion i Endothelial differentiering i hjertets stamceller lavt Serum betingelser

DOI:
10.3791/58370-v

•

12:48 min

•

January 07, 2019

•

, , , ,
1Department of Biomedicine,University Hospital and University of Basel, 2Division of Cardiology,University Hospital Basel

Chapters

  • 00:04Title
  • 01:01Tissue Preparation and Digestion
  • 02:48Filtration
  • 04:43Primary Culture of Isolated SP-CPCs
  • 05:28SP-CPC Growth and Viability Under Different Serum Concentrations
  • 07:28Induction of Endothelial Differentiation
  • 08:59Tube Formation Assay
  • 10:15Results
  • 11:58Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en endotel differentiering teknik for kardiale stamceller. Det fokuserer især på hvordan serum koncentration og celle-seeding tæthed påvirker i endothelial differentiering potentiale.

Tags

Cardiac Progenitor CellsEndothelial DifferentiationLow Serum ConditionsCell TherapyCardiac DiseaseAnesthetizeCollagenase BHank’s Balanced Salt SolutionRed Blood Cell Lysis Buffer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Afledning af Cardiac progenitorceller af embryonale stamceller

Direkte Induktion af humane neurale stamceller fra perifert blod hæmatopoietiske stamceller

Neural Differentiering af Mouse Embryonale stamceller i Serumfrit monolagskultur

Start af Differentiering i Immortaliserede multipotent Otic progenitorceller

Epigenetisk forordning af Cardiac Differentiering af embryonale stamceller og væv

Chondrogene differentiering Induktion af adipøst afledte stamceller ved centrifugal Gravity

Isolation i Endothelial stamceller fra menneskelige navlestrengsblod

In vitro-generering af muse hjerte felt-specifikke kardiale progenitorceller

Panmyeloid differentiering af humant navlestrengsblod afledt CD34+ hæmatopoietisk stamcelle-og progenitorceller

Effektiv differentiering af postganglioniske sympatiske neuroner ved hjælp af humane pluripotente stamceller under Feeder-fri og kemisk definerede kulturbetingelser

Read Article