Journal
/
Immunology and Infection
/
阴离子交换剂 (二乙基氨基乙基纤维素柱) 纯化包括非洲在内的细胞外锥虫体
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
Please note that all translations are automatically generated. Click here for the English version.

阴离子交换剂 (二乙基氨基乙基纤维素柱) 纯化包括非洲在内的细胞外锥虫体

DOI:
10.3791/58415-v

•

14:26 min

•

April 06, 2019

•

, , , , , , , ,
1Univ. Bordeaux UMR INTERTRYP 177 IRD CIRAD, 2IRD UMR 177 INTERTRYP CIRAD, 3CNRS, UMR 5234, Microbiologie Fondamentale et Pathogénicité, Univ. Bordeaux, 4Centre Hospitalier Université Bordeaux

Chapters

  • 00:00Title
  • 01:41Protocol
  • 09:25Results
  • 13:16Conclusion

Summary

Automatic Translation

Automatic Translation

这种从血液中分离锥虫的方法取决于它们的表面电荷比哺乳动物的血细胞更少阴性。受感染的血液被放置在阴离子交换器柱上并进行治疗。这种方法是非洲锥虫病最适合的诊断方法, 为免疫、生物、生化、药物和分子生物学研究提供纯化寄生虫。

Tags

African TrypanosomesSleeping SicknessAnimal African TrypanosomiasisTsetse FlyProtist ParasitesSerologyParasite Concentration TechniquesDEAE CelluloseAnion ExchangerCentrifugationMicroscopic ObservationDiagnosisPurified ParasitesImmunologicalBiologicalBiochemicalPharmaceuticalMolecular Biological InvestigationsPhosphate Buffered SalineGlucosePenicillinStreptomycinPhenol RedPHPotassium Phosphate

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

补消耗的特定细胞群的枯竭

小鼠腹膜腔细胞的分离

特定细胞群的净化荧光激活细胞分选（FACS）

人外周血NK细胞的扩展，纯化和功能评估

热酚水提取纯化及革兰氏阴性菌脂多糖的可视化

病原液泡的分离纯化<em>军团</em>感染吞噬细胞

紧凑型超支化聚乙二醇接枝的膜抗原受保护的功能性红血细胞

生物发光成像检测到非洲锥虫病的晚期感染

葡聚糖增强小鼠和人原发性 NK 细胞的慢转导效率

受肥胖相关炎症组织的巨噬细胞的分离、鉴定和纯化

Read Article