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1Comprehensive Pneumology Center,Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich, 2German Center of Lung Research (DZL), 3Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München,Comprehensive Pneumology Center Munich DZL/CPC-M, 4Department of Experimental Medical Science, Lung Bioengineering and Regeneration,Lund University, 5Wallenberg Center for Molecular Medicine,Lund University, 6Stem Cell Centre,Lund University, 7Asklepios Fachkliniken Munich-Gauting, 8Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine,University of Colorado
Chapters
- 00:04Title
- 00:49Lung Tissue Inflation
- 02:33Precision-cut Lung Slicing (PCLS) and PCLS Punch Generation
- 04:02Results: Representative Experimental 48 h Human 3D-lung Tissue Culture Readouts
- 04:55Conclusion
Qui, presentiamo un protocollo per la preparazione di fette di agarosio-riempito umano del polmone di taglio di precisione da tessuto resecato del paziente che sono adatti per la generazione di colture di tessuti del polmone 3D per malattie polmonari umane modello negli studi biologici e biomedici.
