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Assessing Neuroprotective Effects of Glycyrrhizae Radix et Rhizoma Extract Using a Transient Middle Cerebral Artery Occlusion Mouse Model
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Assessing Neuroprotective Effects of Glycyrrhizae Radix et Rhizoma Extract Using a Transient Middle Cerebral Artery Occlusion Mouse Model

Assessing Neuroprotective Effects of Glycyrrhizae Radix et Rhizoma Extract Using a Transient Middle Cerebral Artery Occlusion Mouse Model

DOI:
10.3791/58454-v

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08:53 min

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December 09, 2018

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, , , , , ,
1School of Korean Medicine,Pusan National University, 2College of Medicine,Dongguk University, 3Kyunghee Naseul Korean Medicine Clinic

Chapters

  • 00:04Title
  • 00:51Glycyrrhizae Radix et Rhizome (GR) Methanol Extract (GRex) Preparation
  • 02:05Mouse Middle Cerebral Artery Occlusion (MCAO) Model
  • 04:10Measurement of Volume of Damaged Brain Tissue
  • 05:02Hematoxylin and Eosin (H&E) and Cresyl Violet Staining
  • 07:18Results: Representative GRex Treatment Effects on MCAO-Injured Mice
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

In this study, we modify an existing experimental method to obtain more reproducible results, by establishing a middle cerebral artery occlusion (MCAO) mouse model. Oral administration of Glycyrrhizae Radix et Rhizome (GR) methanol extract (GRex), following stroke induction, significantly decreased total infarction volume relative to the untreated control group.

Tags

Keywords: NeuroprotectiveGlycyrrhizae Radix Et RhizomaTransient Middle Cerebral Artery OcclusionMouse ModelNatural CompoundsIschemic TimingMiddle Cerebellar ArteryGlycyrrhizae Radix Et Rhizome ExtractMethanol ExtractionFreeze-dryingDimethylsulfoxideC57BL/6 MouseCommon Carotid ArteryExternal Carotid ArteryInternal Carotid ArterySilicone-coated Monofilament

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