/

Developmental Biology

/

成人小鼠成纤维细胞对红细胞生成子细胞的直接线重编程

JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Please note that all translations are automatically generated. Click here for the English version.

成人小鼠成纤维细胞对红细胞生成子细胞的直接线重编程

DOI:

10.3791/58464-v

•

11:46 min

•

December 14, 2018

•

Melissa Ilsley, Sandra Capellera-Garcia, Kishori Dhulipala, Alban Johansson, Johan Flygare

¹Department of Molecular Medicine and Gene Therapy,Lund University

Chapters

00:04Title
01:25Establishment and Maintenance of Primary Mouse Tail Tip Fibroblast Cultures
05:13Retrovirus Production
07:10GTLM Transduction and iEP Harvest
08:52Results: Reprogramming of Induced Erythroid Progenitors (iEPs)
11:17Conclusion

Summary

Automatic Translation

在这里, 我们提出了我们的协议, 产生诱导红血球祖细胞 (iep) 从小鼠成纤维细胞使用转录因子驱动的直接谱系重新编程 (dlr)。

Tags

Direct Lineage Reprogramming Fibroblasts Erythroid Progenitor Cells Transcriptional Regulation In Vitro Tail-tip Fibroblasts Transfusion Medicine Chromatin Modifications Gene Expression Changes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在成年小鼠V-SVZ细胞的神经分析的稳定，高效的基因修饰干细胞自主和非自主的影响

小鼠皮肤成纤维隔离用流式细胞仪

从鼠耳和尾组织生成主成纤维细胞培养

蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎

方法在成纤维细胞迁移的规律研究大分子复合物含MRP4，

重新编程的小鼠胚胎成纤维细胞转录因子诱导Hemogenic计划

评估心肌亚型以下鼠标胚胎成纤维细胞的转录因子介导的重编程

无支架三维胰岛素表达 Pancreatoids 的实验研究体外培养的小鼠胰祖细胞

一种直接而简单的方法，用于评估从胚胎到成人的果蝇黑色素的生存能力

用于微CT成像的成人和早期产后小鼠肺的血管铸造

Read Article