Journal

Biochemistry

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

DOI:

10.3791/58471-v

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05:27 min

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January 26, 2019

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K. Martin Chow, Sidney W. Whiteheart, Jeffrey R. Smiley, Savita Sharma, Kathy Boaz, Meggie J. Coleman, Alvina Maynard, Louis B. Hersh, Craig W. Vander Kooi

¹Department of Molecular and Cellular Biochemistry,University of Kentucky, ²Division of Laboratory Animal Resources,University of Kentucky, ³River Hill Ranch

Chapters

00:04Title
01:39Generation of Antigen-specific Alpaca Antibodies
03:02Purifying Lymphocytes for Single Domain Antibody Library Construction
03:59Results: Single Domain Antibodies Generated against a Range of Protein Antigens in Alpacas (Lama pacos)
04:46Conclusion

Summary

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A method for the production of single domain antibodies from alpacas, including immunization, blood collection, B-cell isolation, and selection is described.

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

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