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Purification of Low-abundant Cells in the Drosophila Visual System
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JoVE Journal Genetics
Purification of Low-abundant Cells in the Drosophila Visual System

Purification of Low-abundant Cells in the Drosophila Visual System

DOI:
10.3791/58474-v

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07:09 min

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September 26, 2018

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, ,
1Department of Neurobiology,Harvard Medical School

Chapters

  • 00:04Title
  • 00:30Papain and Proteolytic Enzyme Blend
  • 01:49Dissections
  • 02:44Cell Dissociation
  • 05:45Results: Effective Purification of Low-abundant Drosophila Cells
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a cell dissociation protocol for efficiently isolating cells present at low abundance within the Drosophila visual system through fluorescence activated cell sorting (FACS).

Tags

Keywords: Cell PurificationDrosophila Visual SystemTranscriptomic AnalysisGenomic AnalysisPapainProteolytic EnzymeOptic LobesMicrotubeDissectionLow-abundant Cells

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