/

/

多平行板流室对剪切应力暴露的内皮细胞基因表达分析

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers

Please note that all translations are automatically generated. Click here for the English version.

多平行板流室对剪切应力暴露的内皮细胞基因表达分析

DOI:

10.3791/58478-v

•

08:50 min

•

October 21, 2018

•

H.S. Jeffrey Man², Aravin N. Sukumar², Kyung Ha Ku³, Michelle K. Dubinsky², Noeline Subramaniam², Philip A. Marsden^2,3,4

¹Institute of Medical Science,University of Toronto, ²Keenan Research Centre in the Li Ka Shing Knowledge Institute,St. Michael’s Hospital, ³Department of Laboratory Medicine and Pathobiology,University of Toronto, ⁴Department of Medicine,University of Toronto

Chapters

00:04Title
00:55Assay Setup
04:20Flow Assay
05:18Cell Harvest and RNA Extraction
06:48Results: Representative KLF2 Loss-of-Function Analysis in Human Endothelial Cells in Response to Shear Stress
08:06Conclusion

Summary

Automatic Translation

本文介绍了流体剪切应力作用下内皮细胞培养和基因表达分析的工作流程。包括一个物理安排, 可同时在受控环境中同时外壳和监测多个流室, 并使用外源参考 RNA 进行定量 PCR。

Tags

Keywords: Endothelial Cells Shear Stress Parallel-plate Flow Chambers Gene Expression Analysis Atherosclerosis Laminar Flow Cell Culture Fibronectin CO2 Incubator Bubble Trap Cell Seeding Tubing Stopcock

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

平行板流动腔和评估内皮祖细胞层流剪应力下连续流电路

高分子基因传递纳米粒子的纳米粒子跟踪分析和高通量流式细胞仪评价

在肌肉再生的老鼠模型中移植诱导多能干细胞衍生的中生代细胞样肌原体

的剪切粘合地图使用SynVivo合成微血管网络代

多步制备技术，以恢复多个代谢化合物的种类进行深入和信息化代谢组学分析

微流体流钱伯斯再造使用血止血建模和血小板输注<em>体外</em

设计微流体装置用于研究细胞反应在单人或共存化工/电气/剪切应力刺激

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

利用自动高通量微流控系统对铜绿假单胞菌剪切应力的活细胞分析

Read Article