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Label-fri Imaging af enkelt proteiner udskilles fra levende celler via iSCAT mikroskopi
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1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning,Milligan College, 3Department of Physics,Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology,Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen
Chapters
- 00:04Title
- 01:18Building the Interferometric Scattering (iSCAT) Microscope
- 06:48iSCAT Microscopy of Secreting Cells
- 08:53Results: Label-Free Detection of Proteins Secreted by a Single Cell
- 09:36Conclusion
Vi præsenterer en protokol til real-time optisk påvisning af enkelt umærket proteiner som de udskilles fra levende celler. Dette er baseret på interferometriske spredning (iSCAT) mikroskopi, som kan anvendes til en række forskellige biologiske systemer og konfigurationer.
Tags
Keywords: Label-free ImagingSingle ProteinsISCAT MicroscopyBiological NanoparticlesExtinction Cross SectionScattering BackgroundHome-built MicroscopeLUZ CellsMolecular-level ProbingOptical SetupDamped Optical TableHigh Numerical Aperture ObjectiveDiode LaserWide-field LensImmersion OilGlass CoverslipAnti-reflection Coated Beamsplitter
