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En Ex Vivo kylling primære Bursal-cellekultur Model til at studere smitsomme Bursal Disease Virus patogenese
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis

En Ex Vivo kylling primære Bursal-cellekultur Model til at studere smitsomme Bursal Disease Virus patogenese

DOI:
10.3791/58489-v

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07:26 min

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October 04, 2018

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, , , ,
1The Pirbright Institute

Chapters

  • 00:04Title
  • 00:45Isolation of Chicken Primary Bursal Cells
  • 02:59Culture of Chicken Primary Bursal Cells
  • 03:51Infection of Chicken Primary Bursal Cells with IBDV and Quantification of IBDV Replication
  • 05:02Results: Analysis of Chicken Primary Bursal Cell Culture and Infection with Infectious Bursal Disease Virus
  • 06:41Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi isolering af kylling primære bursal celler fra bursa af Fabricius, kultur og infektion i cellerne med smitsomme bursal disease virus og kvantificering af viral replikation.

Tags

Ex VivoChickenPrimary Bursal-cell CultureInfectious Bursal Disease VirusPathogenesisAvian VirologyB CellsImmortalized Cell LinesThree RsPBSCollagenase DHBBSRPMIFBSDensity Gradient MediaPolysucroseSodium Diatrizoate

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