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Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
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JoVE Journal Medicine
Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

DOI:
10.3791/58562-v

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06:46 min

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February 15, 2019

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, ,
1Department of Ophthalmology,SUNY Upstate Medical University, 2Department of Dermatology,Columbia University Medical Center

Chapters

  • 00:04Title
  • 00:58Globe Dissection, Wounding, and Cornea Harvest
  • 02:49Cornea Mounting and Transfection
  • 04:42Results: Representative Corneal Wound Healing Analyses
  • 06:06Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for an ex vivo corneal organ culture model useful for wound healing studies is described. This model system can be used to assess the effects of agents to promote regenerative healing or drug toxicity in an organized 3D multicellular environment.

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