Journal
/
Bioengineering
/
High-resolution Patterned Biofilm Deposition Using pDawn-Ag43
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
High-resolution Patterned Biofilm Deposition Using pDawn-Ag43

High-resolution Patterned Biofilm Deposition Using pDawn-Ag43

DOI:
10.3791/58625-v

•

07:47 min

•

October 23, 2018

•

,
1Department of Bioengineering,Stanford University

Chapters

  • 00:04Title
  • 00:57Preparation of pDawn-Ag43 Bacterial Strains and Projector Optical Set-up for Illuminating Bacteria
  • 03:44Culturing Patterned Biofilms
  • 05:18Imaging Patterned Biofilms
  • 06:02Results: High-resolution Deposition of Bacterial Biofilms Using pDawn-Ag43
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate a method for depositing Escherichia coli bacterial biofilms in arbitrary spatial patterns with a high resolution using optical stimulation of a genetically encoded surface-adhesion construct.

Tags

Keywords: Biofilm LithographyPDawn-Ag43Biofilm PatterningOptical SetupBacterial CultureHigh-resolution Biofilm DepositionStructure-function RelationshipBiofilm EngineeringBiomaterialsSynthetic Biology

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58625?article_type=v

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
check_url/58625?article_type=v

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
check_url/58625?article_type=v

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
check_url/58625?article_type=v

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
check_url/58625?article_type=v

Candida albicans Biofilm Chip (CaBChip) for High-throughput Antifungal Drug Screening
check_url/58625?article_type=v

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
check_url/58625?article_type=v

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
check_url/58625?article_type=v

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
check_url/58625?article_type=v

Microfluidic Genipin Deposition Technique for Extended Culture of Micropatterned Vascular Muscular Thin Films
check_url/58625?article_type=v

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

Read Article