Journal
/
Immunology and Infection
/
Etablering av dual Humanisert TK-NOG Mouse modell for HIV-assosiert Liver patogenesen
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

Etablering av dual Humanisert TK-NOG Mouse modell for HIV-assosiert Liver patogenesen

DOI:
10.3791/58645-v

•

00:10 min

•

September 11, 2019

•

, , , ,
1Department of Pharmacology and Experimental Neuroscience,University of Nebraska Medical Center, 2Department of Pathology and Microbiology,University of Nebraska Medical Center

Chapters

  • 00:04Title
  • 00:47Processing of Umbilical Cord Blood and the Isolation of Human HSPCs
  • 04:25Preparation of Human Hepatocytes for Transplantation
  • 05:10Preparation for the Surgery and Instrasplenic Transplantation of Human HSPCs and Hepatocytes in Mice
  • 06:50HIV Infection of TK-NOG Mice and Its Effect on the Human Liver and Immune System
  • 07:48Results: Analysis of the Dual Humanized TK-NOG Mouse Model
  • 09:25Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen gir en pålitelig metode for å etablere humanisert mus med både humant immunsystem og leverceller. Dual rekonstituert immunodeficient mus oppnådd via intrasplenic injeksjon av menneskelig HEPATOCYTTER og CD34+ blodkreft stamceller er mottakelige for humant immunsviktvirus-1 infeksjon og recapitulate leverskader som observert i HIV-infiserte pasienter.

Tags

HIVLiver PathogenesisHumanized Mouse ModelHematopoietic Stem CellsHepatocytesUmbilical Cord BloodCD34 Positive CellsCell SeparationMagnetic Activated Cell Sorting (MACS)Cell CountingCell CultureTransplantation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

In vitro Biofilm Formation in an 8-well Chamber Slide

Bruke BLT humanisert Mouse som en stamcelle basert Gene Therapy Tumor Model

Insektet<em> Galleria mellonella</em> Som en kraftig infeksjon Model for å etterforske Bakteriell Patogenese

Isolering, rensing og merking av benmarg fra mus Nøytrofiler for funksjonelle studier og adoptivforeldre Transfer Eksperimenter

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

Humanisert mus modell for å studere bakterieinfeksjoner Targeting microvasculature

To-fotonet Intravital Imaging av leukocytter under immunrespons i lipopolysakkarid-behandlet musen leveren

Humanized hilsen/SCID/IL2rγnull (hu-NSG) mus modell for HIV replikering og ventetid studier

En Ex Vivo kylling primære Bursal-celle kultur modell å studere Bursal infeksjonssykdommer Virus patogenesen

Evaluere virulens og patogenesen av Aeromonas i en Caenorhabditis elegans modell

Read Article