Journal
/
Biochemistry
/
Isolation of Tissue Extracellular Vesicles from the Liver
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Isolation of Tissue Extracellular Vesicles from the Liver

Isolation of Tissue Extracellular Vesicles from the Liver

DOI:
10.3791/58649-v

•

00:05 min

•

August 21, 2019

•

, ,
1Department of Transplantation,Mayo Clinic

Chapters

  • 00:04Title
  • 00:52Liver Cannulation and Perfusion
  • 03:03Extracellular Vesicle (EV) Isolation
  • 04:30Results: Representative Liver EV Size and Concentration by Nanoparticle Tracking Analysis (NTA)
  • 05:10Conclusion

Summary

Automatic Translation

Automatic Translation

This is a protocol to isolate tissue extracellular vesicles (EVs) from the liver. The protocol describes a two-step process involving collagenase perfusion followed by differential ultracentrifugation to isolate liver tissue EVs.

Tags

Keywords: Extracellular VesiclesLiverPortal Vein CannulationHBSS PerfusionCollagenase Digestion

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58649?article_type=v

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
check_url/58649?article_type=v

Rapid Isolation of the Mitoribosome from HEK Cells
check_url/58649?article_type=v

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
check_url/58649?article_type=v

Imaging of Extracellular Vesicles by Atomic Force Microscopy
check_url/58649?article_type=v

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
check_url/58649?article_type=v

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
check_url/58649?article_type=v

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
check_url/58649?article_type=v

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
check_url/58649?article_type=v

Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages (Video) | JoVE
check_url/58649?article_type=v

Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery (Video) | JoVE

Read Article