Journal
/
Biology
/
En BW Reporter System for å studere reseptor-Ligand interaksjoner
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
A BW Reporter System for Studying Receptor-Ligand Interactions

En BW Reporter System for å studere reseptor-Ligand interaksjoner

DOI:
10.3791/58685-v

•

06:05 min

•

January 07, 2019

•

, , ,
1Department of Hematology,Hadassah – Hebrew University Medical Center, 2The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC),Hadassah – Hebrew University Medical Center

Chapters

  • 00:04Title
  • 00:36Transfection of the Plasmid that Expresses the Chimeric Protein into BW Cells
  • 03:10Incubation of the Transfected BW Cells with Targets
  • 03:32ELISA
  • 04:38Results: BW Reporter System Demonstrated
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver hvordan du oppretter en reporter system som kan brukes til å identifisere og telle reseptor-ligand interaksjoner.

Tags

Keywords: BW Reporter SystemReceptor-ligand InteractionsLigandEndogenous LigandTransfectionPcDNA3 PlasmidSodium AcetateEthanol PrecipitationElectroporationG418 Selection96-well PlateELISA

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58685?article_type=v

Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
check_url/58685?article_type=v

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
check_url/58685?article_type=v

Associated Chromosome Trap for Identifying Long-range DNA Interactions
check_url/58685?article_type=v

Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System
check_url/58685?article_type=v

Grådighet-basert Ekstracellulær Interaction Screening (AVEXIS) for Scalable Detection of Low-affinitet ekstracellulære Receptor-ligand interaksjoner
check_url/58685?article_type=v

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
check_url/58685?article_type=v

Gransker Protein-protein interaksjoner i levende celler ved hjelp Bioluminesens Resonance Energy Transfer
check_url/58685?article_type=v

RAW264.7 transfektere celler med en luciferasereportergenet
check_url/58685?article_type=v

Rask analyse av i døgn fenotyper i Arabidopsis Protoplaster tilført med en selvlysende klokke Reporter
check_url/58685?article_type=v

Studerer Protein Quality Control System of<em> D. discoideum</em> Bruke Temperaturstyrt Live-Cell Imaging

Read Article