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Kvantifisere leukocytter Egress via lymfekar fra Murine huden og svulster
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Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors

Kvantifisere leukocytter Egress via lymfekar fra Murine huden og svulster

DOI:
10.3791/58704-v

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08:39 min

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January 07, 2019

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, , , , ,
1Department of Cell, Developmental, & Cancer Biology,Oregon Health and Science University, 2Department of Molecular Microbiology & Immunology,Oregon Health and Science University, 3Department of Dermatology,Oregon Health and Science University, 4Knight Cancer Institute,Oregon Health and Science University

Chapters

  • 00:04Title
  • 00:46Mouse Pinna Inflammation Induction, Vaccinia Infection, Photoconversion, and Tissue Processing
  • 02:52Intradermal Melanoma Tumor Photoconversion and Tissue Processing
  • 04:00Flow Cytometry Preparation & Analysis
  • 05:18Results: Representative Photoconversion Efficiency in Skin and the Tumor Microenvironments
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

Her viser vi metodene for i vivo kvantifisering av leukocytter egress fra naiv, betent, og ondartet murine hud. Vi utfører en head-to-head sammenligning av to modeller: transdermal FITC programmet og i situ photoconversion. Videre viser vi nytten av photoconversion for å spore leukocytter egress fra kutan svulster.

Tags

Leukocyte EgressLymphatic VesselsMurine SkinTumorsPhotoconversionIn VivoQuantitative AnalysisLeukocyte ResidenceExit DynamicsDisease StatesCutaneous TumorsInflammationKaede Transgenic MouseDibutyl PhthalateVaccinia InfectionLymph NodesCollagenase DDNaseCell Suspension

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