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1Hotchkiss Brain Institute,University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine,University of Calgary, 3Alberta Children’s Hospital Research Institute,University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine,University of Calgary
Chapters
- 00:04Title
- 00:56Dissociating Tissue (Day 1)
- 03:46Isolating Viable and Healthy Cells (Day 1)
- 04:46GEM (Gel Bead in Emulsion) Generation and Barcoding (Day 1)
- 06:17NCBI’s GEO and SRA Submissions
- 07:34Results: Bioinformatics Analysis of Single Cell Transcriptomics
- 09:35Conclusion
Det här protokollet beskriver de allmänna processerna och kvalitetskontroll kontroller som krävs för att förbereda friska vuxna enda däggdjursceller droplet-baserade, hög genomströmning enda cell RNA-Seq preparat. Sekvensering parametrar, finns Läs justering och nedströms encelliga bioinformatiska analyser också.
