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卡瑞津、S100 蛋白和蛋白质基因产物9.5 的免疫修饰直肠吸吮活检诊断

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Neuroscience

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JoVE Journal Neuroscience

Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5

Please note that all translations are automatically generated. Click here for the English version.

卡瑞津、S100 蛋白和蛋白质基因产物9.5 的免疫修饰直肠吸吮活检诊断

DOI:

10.3791/58799-v

•

05:45 min

•

April 26, 2019

•

Shuiqing Chi, Mijing Fang, Kang Li, Li Yang, Shao-tao Tang

¹Department of Pediatric Surgery, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, ²Division of Gastroenterology, Hepatology and Nutrition,Cincinnati Children’s Hospital Medical Center

Chapters

00:04Title
00:40Rectal Suction Biopsy (RSB) Section Preparation
01:52Antigen Retrieval and Tissue Staining
04:25Results: Representative RSB Immunohistochemical Analysis
05:09Conclusion

Summary

Automatic Translation

该方案描述了对卡莱丁、S100 蛋白和蛋白质基因产物9.5 进行免疫染色直肠抽吸活检的过程。这种新型辅助诊断方法对先天性巨结肠病具有较好的敏感性和特异性。

Tags

Keywords: Hirschsprung’s Disease Immunostaining Rectal Suction Biopsy Calretinin S100 Protein Protein Gene Product 9.5 Diagnosis Sensitivity Specificity Paraformaldehyde Dehydration Paraffin Embedding Microtome Antigen Retrieval Hydrogen Peroxide Bovine Serum Albumin Primary Antibody Polymer Enhancer Secondary Antibody

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