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新鲜分离和内联小鼠脑内皮细胞内钙和膜电位的同时测量
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JoVE Journal Biology
Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium
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新鲜分离和内联小鼠脑内皮细胞内钙和膜电位的同时测量

DOI:
10.3791/58832-v

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09:45 min

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January 20, 2019

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,
1Department of Basic Sciences,Loma Linda University

Chapters

  • 00:04Title
  • 00:48Dissection and Isolation of Cerebral Artery
  • 02:34Preparation of Endothelial Tube and Superfusion
  • 05:44Simultaneous Measurement of [Ca2+]i and Vm
  • 08:36Results: Simultaneous [Ca2+]i and Vm Recordings
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

这里演示的是 (1) 新鲜隔离完整的大脑内皮 "管" 和 (2) 同时测量内皮钙和膜电位的协议, 在内皮衍生的超极化过程中。此外, 这些方法允许药理调节内皮细胞钙和电信号作为个人或交互式实验变量。

Tags

Intracellular CalciumMembrane PotentialCerebral EndotheliumCerebral Vascular PhysiologyCerebral Blood Flow RegulationAgingChronic DiseasesMouse Brain IsolationCerebral Artery IsolationEnzymatic DigestionSimultaneous MeasurementPhysiological Conditions

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