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在成细胞分化和融合过程中利用活成像跟踪核

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Biology

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Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion

Please note that all translations are automatically generated. Click here for the English version.

在成细胞分化和融合过程中利用活成像跟踪核

DOI:

10.3791/58888-v

•

09:03 min

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April 13, 2019

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Giorgia Careccia*^1,2, Federica Colombo*^1,3, Mario Tirone, Alessandra Agresti, Marco E. Bianchi², Samuel Zambrano*^1,2, Emilie Vénéreau*¹

¹Chromatin Dynamics Unit,Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) San Raffaele Scientific Institute, ²Vita-Salute San Raffaele University, ³Department of Electronics, Information and Bioengineering,Politecnico di Milano

Chapters

00:00Title
00:43Primary Myoblast Isolation
03:30Myoblast Proliferation and Differentiation
06:25Results: Representative Myoblast Nucleus Differentiation and Tracking Analyses
08:14Conclusion

Summary

Automatic Translation

骨骼肌分化是一个高度动态的过程, 它特别依赖于核定位。在这里, 我们描述了一种方法, 以跟踪核运动的活细胞成像在成肌细胞分化和肌管形成, 并执行核动力学的定量表征, 从自动跟踪提取信息。

Tags

Live Cell Imaging Myoblast Differentiation Myofiber Formation Nuclear Positioning Primary Cell Isolation Cell Tracking Muscle Cell Isolation Satellite Cell Isolation

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