A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics,Nanjing University of Chinese Medicine, 2TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory,Nanjing University of Chinese Medicine, 3Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing University of Chinese Medicine, 4Department of Traumatology and Orthopedics,Affiliated Hospital of Nanjing University of Chinese Medicine
Chapters
- 00:04TItle
- 00:55Traditional Method of Isolating Bone Marrow-derived Cells
- 02:40Improved Method of Isolating Bone Marrow-derived Cells
- 04:11Purification by Density Gradient Centrifugation
- 05:39Culture and Differentiation
- 06:15Bone Resorption Assay Using Toluidine Blue Staining
- 08:03Scanning Electron Microscopy
- 09:03Results: Analysis of the Osteoclast Precursors Isolated, Purified, and Differentiated from Rat Bone Marrow
- 10:40Conclusion
Osteoklaster er vævsspecifikke makrofag polykaryoner afledt af monocyte-makrofag-Lineage af hæmatopoietiske stamceller. Denne protokol beskriver, hvordan man isolerer knoglemarvsceller, så der opnås store mængder osteoklaster, samtidig med at risikoen for ulykker, der findes i traditionelle metoder, mindskes.
