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Biochemistry
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电化学和荧光显微镜在质子泵膜酶研究中的单脂质体测定
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Biochemistry
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JoVE Journal
Biochemistry
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
电化学和荧光显微镜在质子泵膜酶研究中的单脂质体测定
DOI:
10.3791/58896-v
•
12:15 min
•
February 21, 2019
•
Ievgen Mazurenko
,
Nikos S. Hatzakis
,
Lars J.C. Jeuken
1
School of Biomedical Sciences & the Astbury Centre for Structural Molecular Biology
,
University of Leeds
,
2
Department of Chemistry and Nano-Science Center
,
University of Copenhagen
Chapters
00:04
Title
00:28
Preparation of a Lipid/UQ-10/FDLL Mix
01:26
Reconstitution of Cytochrome bo
3
05:06
Fabrication of Semi-Transparent Gold Electrodes and Modification of the Gold Surface with Self-Assembled Monolayer (SAM)
06:07
Electrochemical Testing of Proteoliposomes Activity
07:46
Detection of Enzymatic Proton Pumping by Fluorescence Microscopy
09:44
Results: Liposome Identification and pH Change
11:35
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个方案, 研究质子在单个脂质体脂质膜上的分子转移机制, 以细胞色素
b3
为例。结合电化学和荧光显微镜, 可以单独检测和分析含有单一或多种酶的单囊腔内的 ph 值变化。
Tags
Keywords: Single Liposome
Proton-pumping
Membrane Enzymes
Electrochemistry
Fluorescent Microscopy
Cytochrome BO3
Escherichia Coli
Ubiquinone 10
FDLL
MOPS Buffer
HPTS
OGP Surfactant
Polystyrene Microbeads
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