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电化学和荧光显微镜在质子泵膜酶研究中的单脂质体测定
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
DOI:

12:15 min

February 21, 2019

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Chapters

  • 00:04Title
  • 00:28Preparation of a Lipid/UQ-10/FDLL Mix
  • 01:26Reconstitution of Cytochrome bo3
  • 05:06Fabrication of Semi-Transparent Gold Electrodes and Modification of the Gold Surface with Self-Assembled Monolayer (SAM)
  • 06:07Electrochemical Testing of Proteoliposomes Activity
  • 07:46Detection of Enzymatic Proton Pumping by Fluorescence Microscopy
  • 09:44Results: Liposome Identification and pH Change
  • 11:35Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个方案, 研究质子在单个脂质体脂质膜上的分子转移机制, 以细胞色素b3 为例。结合电化学和荧光显微镜, 可以单独检测和分析含有单一或多种酶的单囊腔内的 ph 值变化。

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