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Genetics
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非人类灵长类动物造血干细胞和祖细胞的制备及基因修饰
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Genetics
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Genetics
Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
Please note that all translations are automatically generated.
Click here for the English version.
非人类灵长类动物造血干细胞和祖细胞的制备及基因修饰
DOI:
10.3791/58933-v
•
11:16 min
•
February 15, 2019
•
Stefan Radtke
,
Anai M. Perez
,
Rasika Venkataraman
,
Sowmya Reddy
,
Kevin G. Haworth
,
Olivier Humbert
,
Hans-Peter Kiem
2,3
,
Christopher W. Peterson
2
1
Stem Cell and Gene Therapy Program
,
Fred Hutchinson Cancer Research Center
,
2
Department of Medicine
,
University of Washington
,
3
Department of Pathology
,
University of Washington
Chapters
00:04
Title
00:42
Bone Marrow Hemolysis (Day -1)
02:44
CD34-enriched Cell Quality Control (Day -1)
05:10
CD34+ Hematopoietic Stem and Progenitor Cell (HSPC) Gene Modification and Overnight Recovery (Day 0)
06:33
Cell Harvest and Infusion Preparation (Day 1)
07:42
Liquid Culture Quality Control (Days 1-5)
09:19
Results: Representative HSPC Identification and Analysis
10:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案的目标是从启动的骨髓中分离出非人类灵长类动物 cd34
+
细胞, 用慢病毒载体对这些细胞进行基因修饰, 并准备一种产品, 用于注入自体宿主。协议的总长度约为48小时。
Tags
Nonhuman Primate
Hematopoietic Stem Cells
Progenitor Cells
Gene Therapy
Bone Marrow
Cell Isolation
CD34
Magnetic Cell Sorting
Flow Cytometry
Cell Culture
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