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Identifisere Cell Surface markører for primær neural Stem og stamceller ved metabolsk merking av Sialoglycan
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1Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology,Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education, 2College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University
Chapters
- 00:05Title
- 01:16Preparation of Mouse Endothelial Culture in Permeable Support Inserts
- 01:55Set-up of Neural-endothelial Co-culture and Ac4ManNAz Labeling System
- 03:25Immunofluorescent Staining of Sialoglycoproteins in Expanded Primary NSPCs and Differentiated Neurons
- 05:25Purification of Sialoglycoproteins from Expanded Primary NSPCs and Differentiated Neurons
- 08:45Results: Identifying Cell Surface Markers by Metabolic Labeling of Sialoglycan
- 10:47Conclusion
Presentert her er en protokoll som kombinerer en in vitro neural-endothelial co-kultur system og metabolsk innlemmelse av sialoglycan med bioorthogonal funksjonelle grupper for å utvide primære nevrale stammen og stamceller og merke deres overflate sialoglycoproteins for bildebehandling eller masse-massespektrometri analyse av celleoverflaten markører.
