Journal
/
Immunology and Infection
/
Merkel Cell Polyomavirus Infection and Detection
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Merkel Cell Polyomavirus Infection and Detection

Merkel Cell Polyomavirus Infection and Detection

DOI:
10.3791/58950-v

•

13:45 min

•

February 07, 2019

•

, , ,
1Department of Microbiology, Perelman School of Medicine,University of Pennsylvania, 2Laboratory of Cellular Oncology,National Cancer Institute

Chapters

  • 00:04Title
  • 00:42Isolation of Human Dermal Fibroblasts
  • 02:11Recombinant MCPyV Virion Preparation
  • 05:49Infection
  • 07:09In situ DNA-HCR
  • 10:56Results: Infection and Detection of Merkel Cell Polyomavirus in Human Dermal Fibroblasts
  • 12:45Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to infect primary human dermal fibroblast with MCPyV. The protocol includes isolation of dermal fibroblasts, preparation of MCPyV virions, virus infection, immunofluorescence staining, and fluorescence in situ hybridization. This protocol can be extended for characterizing MCPyV-host interactions and discovering other cell types infectable by MCPyV.

Tags

Merkel Cell PolyomavirusMCPyVCell Culture ModelDermal FibroblastsVirus IsolationImmunofluorescent StainingFluorescent In Situ HybridizationInfectious CycleHost Cell Interactions293TT28 CellsTransfectionST ExpressionLT Expression

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58950?article_type=v

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
check_url/58950?article_type=v

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
check_url/58950?article_type=v

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
check_url/58950?article_type=v

A Visual Assay to Monitor T6SS-mediated Bacterial Competition
check_url/58950?article_type=v

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
check_url/58950?article_type=v

Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry
check_url/58950?article_type=v

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
check_url/58950?article_type=v

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
check_url/58950?article_type=v

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
check_url/58950?article_type=v

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Read Article