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心脏细胞治疗中干细胞 dna 完整性的评估

JoVE Journal
Genetics

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JoVE Journal Genetics

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

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心脏细胞治疗中干细胞 dna 完整性的评估

DOI:

10.3791/58971-v

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10:16 min

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January 25, 2019

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Jessica M. Miller*¹, Nikhil Maneesh Mardhekar*¹, Vasanthi Rajasekaran, Jianyi Zhang, Ram Kannappan

¹Department of Biomedical Engineering, School of Engineering, School of Medicine,University of Alabama at Birmingham

Chapters

00:04Title
00:50Comet Assay
03:19DNA Damage Response
06:12Counting of the DNA Damage Response (DDR) Foci
08:11Results: DNA Damage Response in Human iPS Cells and iPS-derived Cardiomyocytes
09:43Conclusion

Summary

Automatic Translation

在这里, 我们提供了一个实验设置的详细描述, 用于分析细胞移植前干细胞 dna 完整性的评估。

Tags

DNA Integrity Stem Cell Quality Assessment Comet Assay DNA Damage Regenerative Therapy Cardiomyopathy Induced Pluripotent Stem Cells Reactive Oxygen Species Cell Transplantation DNA-RNA Free Water Agarose Comet Slide Lysis Buffer Alkaline Solution Electrophoresis Ethanol

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