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Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention
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Chapters
- 00:04Title
- 00:45On-Chip Immobilization
- 02:22Collection of Animals from a Worm Sheet
- 02:53Application of Worm Sheets for Imaging Observations
- 03:11Treatment of Worm Sheets for Repeated Use
- 03:52Results: C. elegans Individuals Enclosed with PS Film Display No Reduction in Motility
- 04:20Conclusion
A series of immobilization methods has been established to allow the targeted irradiation of live Caenorhabditis elegans individuals using a recently developed ultra-thin polydimethylsiloxane microfluidic chip with water retention. This novel on-chip immobilization is also adequate for imaging observations. The detailed treatment and application examples of the chip are explained.
