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En musemodel at vurdere medfødte immunrespons for Staphylococcus aureus infektioner
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection

En musemodel at vurdere medfødte immunrespons for Staphylococcus aureus infektioner

DOI:
10.3791/59015-v

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09:15 min

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February 28, 2019

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, , , ,
1Department of Biomedical Engineering,University of California Davis, 2Department of Dermatology,Johns Hopkins University School of Medicine

Chapters

  • 00:04Title
  • 00:38Bacterial Preparation and Quantification
  • 02:44Excisional Skin Wounding
  • 03:50Staphylococcus aureus Inoculation
  • 04:44In Vivo Bioluminescent and Fluorescence Imaging
  • 06:02Bioluminescent and Fluorescence Image Analysis
  • 06:45Results: LysM-EGFPMyD88-/- Mice are More Susceptible to S. aureus Infection Than LysM-EGFP Mice
  • 08:30Conclusion

Summary

Automatic Translation

Automatic Translation

En tilgang er beskrevet til real-time opdagelse af medfødte immun respons til kutane såret og Staphylococcus aureus infektion af mus. Ved at sammenligne LysM-EGFP mus (som besidder fluorescerende neutrofiler) med en LysM-EGFP krydset immundefekte mus stamme, vi fremme vores forståelse af infektion og udvikling af metoder til at bekæmpe infektion.

Tags

Mouse ModelStaphylococcus Aureus InfectionInnate Immune ResponseBioluminescenceBacterial BurdenNeutrophil ResponseLongitudinal MonitoringExcisional Skin WoundingBacterial InoculationColony-forming Units

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