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Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions
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Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

DOI:
10.3791/59016-v

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04:33 min

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March 12, 2019

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,
1Graduate Institute of Medicine, College of Medicine,Kaohsiung Medical University, 2Department of Anatomy, School of Medicine,Kaohsiung Medical University, 3Department of Cell Biology and Anatomy, College of Medicine,National Cheng Kung University

Chapters

  • 00:04Title
  • 00:39Adrenal Gland Isolation
  • 01:52Adrenocortical Cell Preparation and Culture
  • 03:26Results: Rat Adrenocortical Cells Analysis and Assays of Steroidogenic Functions
  • 04:06Conclusion

Summary

Automatic Translation

Automatic Translation

The hormone secreted by the adrenal cortex is vital for animals against stress and diseases. Here, we present a protocol to culture the primary rat adrenal cells. It could be a good in vitro platform for investigating the mechanisms of the reagent of interest in adrenal steroidogenesis and lipid biosynthesis.

Tags

Primary Adrenal Cell CultureAdrenocortical CellsSteroidogenesisSteroidogenic FunctionCollagenase DigestionCell IsolationCell PlatingCell Culture Conditions

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