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Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
This content is Free Access.
Chapters
- 00:04Title
- 00:45Creation of a Diffraction Plan in ISPyB
- 03:55Data Collection, Viewing, and Retrieval
- 05:02Results: X-ray Diffraction Analysis of Human Mitochondrial Glycine Cleavage System protein H (GCSH)
- 06:38Conclusion
Here, we describe how to use the automated screening and data collection options available at some synchrotron beamlines. Scientists send cryocooled samples to the synchrotron, and the diffraction properties are screened, the data sets are collected and processed and, where possible, a structure solution is carried out—all without human intervention.
